Metallo-β-lactamases— new therapeutic challenge

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Metallo-β-Lactamases and Aptamer-Based Inhibition

An evolution of antibiotic-resistant bacteria has resulted in the need for new antibiotics. β-Lactam based drugs are the most predominantly prescribed antibiotics to combat bacterial infections; however, production of β-lactamases, which catalyze the hydrolysis of the β-lactam bond of this class of antibiotics, by pathogenic bacteria such as Bacillus cereus, are rendering them useless. Some inh...

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The Mechanisms of Catalysis by Metallo β-Lactamases

Class B beta-lactamases or metallo-beta-lactamases (MBLs) require zinc ions to catalyse the hydrolysis of beta-lactam antibiotics such as penicillins, cephalosporins, carbapenems, and cephamycins. There are no clinically useful inhibitors against MBLs which are responsible for the resistance of some bacteria to antibiotics. There are two metal-ion binding sites that have different zinc ligands ...

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Assay Platform for Clinically Relevant Metallo-β-lactamases

Metallo-β-lactamases (MBLs) are a growing threat to the use of almost all clinically used β-lactam antibiotics. The identification of broad-spectrum MBL inhibitors is hampered by the lack of a suitable screening platform, consisting of appropriate substrates and a set of clinically relevant MBLs. We report procedures for the preparation of a set of clinically relevant metallo-β-lactamases (i.e....

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The New β-Lactamases

From the Department of Infectious Diseases, Lahey Clinic, Burlington, Mass., and Harvard Medical School, Boston (G.A.J.); and the Department of Geographic Medicine and Infectious Diseases, Tufts–New England Medical Center and Tufts University School of Medicine, Boston (L.S.M.-P.). Address reprint requests to Dr. Jacoby at Lahey Clinic, 41 Mall Rd., Burlington, MA 01805, or at george.a.jacoby@l...

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Interaction of Avibactam with Class B Metallo-β-Lactamases

β-Lactamases are the most important mechanisms of resistance to the β-lactam antibacterials. There are two mechanistic classes of β-lactamases: the serine β-lactamases (SBLs) and the zinc-dependent metallo-β-lactamases (MBLs). Avibactam, the first clinically useful non-β-lactam β-lactamase inhibitor, is a broad-spectrum SBL inhibitor, which is used in combination with a cephalosporin antibiotic...

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ژورنال

عنوان ژورنال: Journal of Medical Microbiology

سال: 1993

ISSN: 0022-2615,1473-5644

DOI: 10.1099/00222615-39-2-93